Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

To confirm or not to confirm (microarray data)--that is the question.

John C Rockett1

  • 1Reproductive Toxicology Division (MD-72), National Health and Environmental Effects Research Laboratory, US EPA, Research Triangle Park, NC 27711, USA. rockett.john@epa.gov

Drug Discovery Today
|April 12, 2003
PubMed
Summary

This letter addresses challenges in confirming microarray data after hybridization. It highlights the need for robust validation methods in gene expression studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of rat and human steroidogenesis by triazole antifungals.

Systems biology in reproductive medicine·2009
Same author

Effects of storage, RNA extraction, genechip type, and donor sex on gene expression profiling of human whole blood.

Clinical chemistry·2007
Same author

Success and failure in human spermatogenesis as revealed by teratozoospermic RNAs.

Human molecular genetics·2007
Same author

Biomarkers of reproductive toxicity.

Cancer biomarkers : section A of Disease markers·2006
Same author

Biomarkers of ovulation, endometrial receptivity, fertilisation, implantation and early pregnancy progression.

Paediatric and perinatal epidemiology·2006
Same author

Disruption of testosterone homeostasis as a mode of action for the reproductive toxicity of triazole fungicides in the male rat.

Toxicological sciences : an official journal of the Society of Toxicology·2006

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Microarray technology is widely used for gene expression profiling.
  • Post-hybridization analysis requires careful validation to ensure data accuracy.
  • Confirmatory studies are crucial for reliable biological interpretation.

Purpose of the Study:

  • To discuss critical issues in post-hybridization confirmatory studies of microarray data.
  • To emphasize the importance of rigorous validation in microarray experiments.
  • To highlight potential pitfalls and best practices in data confirmation.

Main Methods:

  • Discussion of common confirmatory techniques.
  • Analysis of potential sources of error in validation.
  • Review of best practices for data integrity.

Related Experiment Videos

Main Results:

  • Identification of key challenges in microarray data validation.
  • Emphasis on the need for standardized confirmatory protocols.
  • Recognition of the impact of validation on downstream analysis.

Conclusions:

  • Robust confirmatory studies are essential for the reliability of microarray data.
  • Addressing these issues will improve the quality and reproducibility of gene expression research.
  • Further development of validation strategies is warranted.