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Molecular cloning and expression analysis of the mouse Spot-2 gene in pituitary development
Toshinobu Miyamoto1, Shiga Hasuike, Kazuo Sengoku
1Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa, 078-8510, Hokkaido, Japan. toshim@asahikawa-med.ac.jp
Abstract:
Mouse Spot-1 is a DNA-binding protein with a domain (His-Thr) encoded by p(CA)n repeats. Spot-1 interacts with the nuclear localization signal (NLS) I of p53 through its His-Thr domain. In this study we describe the cloning and expression patterns of a novel gene encoding a protein containing a His-Thr domain, Spot-2. Spot-2 is exclusively expressed in the pituitary from stage E13.5 to E15.5. Mouse Lhx3 plays a critical role during early organogenesis in the pituitary. The Spot-2 gene appears to be a downstream gene of Lhx3. It is suggested that Spot-2 plays important roles in pituitary development.