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A defect in a fatty acyl-CoA synthetase gene, lcf1+, results in a decrease in viability after entry into the
1Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, 464-8601 Nagoya, Japan.
Molecular Genetics and Genomics : MGG
|April 10, 2003
Summary
A Schizosaccharomyces pombe mutant lacking the lcf1+ gene rapidly loses viability in stationary phase. This long-chain fatty acyl-CoA synthetase is crucial for cell survival under stress conditions.
Area of Science:
- Cellular Biology
- Molecular Genetics
- Biochemistry
Background:
- Schizosaccharomyces pombe is a model organism for studying eukaryotic cell biology.
- Cellular viability during stationary phase is critical for microbial survival.
- Long-chain fatty acyl-CoA synthetases are enzymes involved in lipid metabolism.
Purpose of the Study:
- To investigate the function of the lcf1+ gene in Schizosaccharomyces pombe.
- To characterize a mutant defective in stationary phase viability.
- To understand the role of long-chain fatty acyl-CoA synthetase in cellular stress response.
Main Methods:
- Isolation and characterization of a Schizosaccharomyces pombe mutant.
- Phenotypic analysis including viability assays, temperature sensitivity, and salt tolerance.
- Gene identification through mutational lesion analysis.
- Homology comparison with related genes in Saccharomyces cerevisiae.
Main Results:
- A mutant defective in stationary phase viability was identified.
- The defect was mapped to a mutation in the lcf1+ gene, encoding a long-chain fatty acyl-CoA synthetase.
- The lcf1Δ mutant exhibited sensitivity to high temperature (37°C) and high salt concentrations (0.9 M KCl).
- The mutant cells grew normally during the logarithmic phase but rapidly lost viability upon entering stationary phase.
Conclusions:
- The lcf1+ gene is essential for maintaining viability in stationary phase in Schizosaccharomyces pombe.
- Lcf1p likely plays a role in managing endogenous acyl-CoA pools, contributing to stress tolerance.
- The findings suggest a conserved function of long-chain fatty acyl-CoA synthetases in cellular homeostasis and stress response across yeast species.
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