Flow injection chemiluminescent assays for glycerol and triglycerides using a co-immobilized enzyme reactor

A flow injection method for the determination of glycerol using a co-immobilized enzyme reactor containing glycerokinase and glycerol-3-phosphate oxidase is described. The hydrogen peroxide produced is monitored by using a luminol chemiluminescence reaction in the presence of catalyst such as Co(II). The detection limit (2.5 x blank noise) for glycerol is 7 x 10(-3) mmol/L with a sample throughput of 40/h. The calibration graph is linear over the range studied (0.2-1.0 mmol/L) with relative standard deviation 1.2-2.4%. The method is applied to the determination of glycerol in blood serum produced off-line from triglycerides using lipase isolated from bovine pancreas.