Cellular localization of thromboxane synthase in ovine spinal cord and hindbrain

We and others have demonstrated that endogenously-produced prostanoids modify the function of the hypothalamus-pituitary-adrenal (HPA) axis. We have demonstrated that exogenously-administered thromboxane mimetic stimulates ACTH secretion in fetal sheep, and that the endogenous production of thromboxane modifies the HPA response to cardiovascular stress. The purpose of this study was to identify the structures within the fetal and adult ovine medulla and hindbrain which express immunoreactive thromboxane synthase. Using immunohistochemical techniques, we demonstrated thromboxane synthase immunostaining in regions important for cardiovascular afferent signaling (nucleus tractus solitarius, ventrolateral medulla) in both cell bodies and axons. Thromboxane synthase was also apparent in neuroanatomical locations which are consistent with afferent and efferent projections from the cerebellum. We observed staining in the superior cerebellar peduncle in the rostal pons, in the corticopontocerebellar fibers, and in Purkinje cells. The enzyme was found in motor regions, including the dorsal motor nucleus of the vagus nerve, and in the motor neurons of the dorsal column of the spinal cord. In addition to the apparent neuronal staining, there was positive staining in the ventricular ependymal cells. We conclude that, consistent with physiological evidence, thromboxane synthase is present in brain regions which are important for afferent and efferent cardiovascular signaling.