Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fetal tissue engineering from amniotic fluid.

Amir Kaviani1, Kristine Guleserian, Tjörvi E Perry

  • 1Department of Surgery, Children's Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA.

Journal of the American College of Surgeons
|April 15, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transamniotic Fetal Delivery of Cystic Fibrosis (CF) Transmembrane Conductance Regulator (CFTR) mRNA.

FASEB bioAdvances·2026
Same author

Esophagus engineering tackles function.

Nature biotechnology·2026
Same author

Postnatal Fate of Donor Hematopoietic Stem Cells After Transamniotic Stem Cell Therapy.

The Journal of surgical research·2026
Same author

Self-Amplifying mRNA Enhances Transamniotic Fetal mRNA Delivery.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

A Preoperative Vascular Imaging Protocol for Planning of Supercharged Jejunal Conduits in Pediatric Esophageal Replacement.

Plastic and reconstructive surgery·2026
Same author

Native amniotic fluid mesenchymal stem cell response in a model of fetal growth restriction.

Cytotherapy·2026
Same journal

Breaking the Polytrauma-Brain Barrier: Using Point-of-Care Biomarkers in Severely Injured Trauma Patients.

Journal of the American College of Surgeons·2026
Same journal

Going the Extra Mile: Picking the Right Trauma Center Destination for Critically Injured Patients in a Mature State-Wide Trauma System.

Journal of the American College of Surgeons·2026
Same journal

What Does It Mean for Surgeons to Be Flourishing?

Journal of the American College of Surgeons·2026
Same journal

Tailor-Made Solution to Trimming Venous Thromboembolism Risk.

Journal of the American College of Surgeons·2026
Same journal

NIH Funding in Surgical Artificial Intelligence: Who, What, Where, Why.

Journal of the American College of Surgeons·2026
Same journal

Efficacy and Safety of Rezūm Water Vapor Thermal Ablation in Large and Small Prostates: A Multicenter Comparative Analysis of 2,725 Patients.

Journal of the American College of Surgeons·2026
See all related articles

Human amniotic fluid contains mesenchymal stem cells suitable for fetal tissue engineering. These cells can be cultured and attached to scaffolds, offering a practical source for regenerative medicine applications.

Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Stem Cell Biology

Background:

  • Amniotic fluid previously shown to be a cell source in animal models.
  • Investigating human amniotic fluid cells for fetal tissue engineering.

Purpose of the Study:

  • Determine if human amniotic fluid cells can engineer fetal tissue constructs.
  • Characterize subpopulations of amniotic fluid cells.

Main Methods:

  • Isolated and cultured amniotic fluid cells (n=6, 15-19 weeks gestation).
  • Mechanically isolated and expanded a distinct subpopulation (amniocytes).
  • Determined cell lineage via immunofluorescence and proliferation rates.
  • Seeded amniocytes onto polyglycolic acid (PGA) and acellular human dermis scaffolds.

Related Experiment Videos

  • Analyzed constructs using scanning electron microscopy.

    • Main Results:

    • Amniocytes identified as mesenchymal, fibroblast-myofibroblast lineage.
    • Rapid expansion of mesenchymal amniocytes in culture demonstrated.
    • Dense cell layers and firm adhesion to PGA and Alloderm scaffolds observed.
    • No cell death detected in engineered constructs.

    Conclusions:

    • Mesenchymal cell subpopulations consistently isolated from human amniotic fluid.
    • Rapid in vitro expansion and firm scaffold attachment confirmed.
    • Human amniotic fluid is a valuable and practical cell source for fetal tissue engineering.