Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-resolution confocal imaging and three-dimensional rendering.

Yee-Chien Liu1, Ann-Shyn Chiang

  • 1Department of Life Science, National Tsing Hua University, Hsinchu 30043, Taiwan.

Methods (San Diego, Calif.)
|April 16, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Individualized decision-making driven by long-term memory retrieval in Drosophila.

Cell reports·2026
Same author

Self-Supervised Isotropic Resolution Enhancement of Expansion Microscopy via Quantized Compression.

Journal of imaging informatics in medicine·2026
Same author

Redox regulation of memory formation by Rrp1 in <i>Drosophila</i>.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Thirst-driven hygrosensory suppression promotes water seeking in <i>Drosophila</i>.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Author Correction: BigNeuron: a resource to benchmark and predict performance of algorithms for automated tracing of neurons in light microscopy datasets.

Nature methods·2024
Same author

Asymmetric neurons are necessary for olfactory learning in the Drosophila brain.

Current biology : CB·2024
Same journal

Quantitative single-cell analysis of PML-RARα oncogene-induced DNA damage along cell cycle progression.

Methods (San Diego, Calif.)·2026
Same journal

Cilia SubQ: a modular suite of semi- and fully automated pipelines for analysis of primary cilia and ciliary subdomains.

Methods (San Diego, Calif.)·2026
Same journal

Projective invariant of surface ratio: application to pupil measurement through simulations and proof-of-concept recordings.

Methods (San Diego, Calif.)·2026
Same journal

A quantitative radiographic framework for longitudinal monitoring of additively manufactured biodegradable scaffolds with graded tantalum reinforcement.

Methods (San Diego, Calif.)·2026
Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
See all related articles

New confocal microscopy techniques enable high-resolution imaging of thick biological tissues. FocusClear technology and advanced processing allow visualization of cellular structures and gene expression in whole-mount tissues up to 1mm thick.

Area of Science:

  • Biomedical Engineering
  • Microscopy
  • Cell Biology

Background:

  • Biological tissues' opacity hinders high-resolution confocal imaging.
  • Efficient light penetration and signal detection are crucial for thick tissue analysis.

Purpose of the Study:

  • To present technical advances for high-resolution confocal imaging of intact whole-mount thick tissues.
  • To enable visualization of cellular structures and gene expression within these tissues.

Main Methods:

  • FocusClear technology for tissue transparency and improved light penetration.
  • A universal fluorescence staining method for nuclei and membranes.
  • Post-recording image processing for 3D visualization, segmentation, and volume rendering.

Related Experiment Videos

Main Results:

  • Achieved high-resolution confocal imaging of whole-mount tissues up to 1mm thick.
  • Demonstrated visualization of cellular structures and gene expression at single-cell resolution.
  • Enabled segmentation and volume rendering of regions of interest in whole-mount brains.

Conclusions:

  • Technical advances in confocal microscopy facilitate detailed imaging of thick tissues.
  • These methods offer a resolution comparable to single-cell imaging.
  • The techniques have practical applications in biological research, with discussed limitations.