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Updated: Jul 7, 2026

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
14:53

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Multifunctional gold nanoparticle-peptide complexes for nuclear targeting.

Alexander G Tkachenko1, Huan Xie, Donna Coleman

  • 1Department of Chemistry, North Carolina State Univ, Raleigh, North Carolina 27695, USA.

Journal of the American Chemical Society
|April 17, 2003
PubMed
Summary

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Researchers explored peptide-modified gold nanoparticles for targeting HepG2 cell nuclei. Combining nuclear localization signal (NLS) and receptor-mediated endocytosis (RME) peptides on nanoparticles improved nuclear delivery, especially when peptides were separate.

Area of Science:

  • Nanotechnology
  • Cell Biology
  • Biomedical Engineering

Background:

  • Gold nanoparticles offer a versatile platform for developing multifunctional nanostructured materials.
  • Targeting specific cellular compartments, like the nucleus, is crucial for advanced diagnostics and therapeutics.
  • Previous attempts with solely nuclear localization signal (NLS) peptides failed to effectively deliver nanoparticles into HepG2 cell nuclei.

Purpose of the Study:

  • To investigate the efficacy of peptide-modified gold nanoparticles in targeting the nucleus of HepG2 cells.
  • To compare the nuclear targeting efficiency of different peptide combinations and configurations on nanoparticle surfaces.
  • To evaluate the role of nuclear localization signal (NLS) and receptor-mediated endocytosis (RME) peptides in nanoparticle cellular uptake and nuclear delivery.

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Related Experiment Videos

Last Updated: Jul 7, 2026

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
14:53

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Determining the Mechanical Strength of Ultra-Fine-Grained Metals
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Determining the Mechanical Strength of Ultra-Fine-Grained Metals

Published on: November 22, 2021

Main Methods:

  • Synthesis and characterization of gold nanoparticles modified with five distinct peptide complexes.
  • Utilized video-enhanced color differential interference contrast microscopy for nanoparticle visualization.
  • Assessed nanoparticle entry into HepG2 cells and subsequent nuclear localization based on peptide modifications.

Main Results:

  • Nanoparticles modified solely with NLS peptides did not effectively target the cell nucleus, often being endosomally trapped.
  • A combination of NLS and RME peptides on nanoparticles facilitated nuclear entry into HepG2 cells.
  • Nuclear targeting was significantly more efficient when NLS and RME peptides were attached as separate short peptides compared to a single long peptide.

Conclusions:

  • Effective nuclear targeting of HepG2 cells requires a combination of endocytosis-promoting and nuclear localization signals.
  • The spatial arrangement of functional peptides on nanoparticle surfaces critically influences intracellular delivery efficiency.
  • Multifunctional nanostructured materials hold promise as advanced tools for intracellular diagnostics and therapeutic delivery.