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Polymorphism of the interleukin-1beta gene and endometriosis
Fritz Wieser1, Lukas Hefler, Clemens Tempfer
1Division of Gynecological Endocrinology and Reproductive Medicine, Department of Obstetrics and Gynecology, University of Vienna, Vienna, Austria.
Objective:
Experimental data indicate that interleukin (IL)-1 plays a role in the pathogenesis of endometriosis. We determined the genotype and the allele frequencies of the IL1B exon 5 polymorphism and the corresponding IL-1beta serum levels in women with endometriosis.
Methods:
We genotyped 92 women with surgically and histologically confirmed endometriosis and 69 controls without history of endometriosis. Both groups were of middle European genetic background for the IL1B exon 5 polymorphism (at position +3954). Genotyping was performed by pyrosequencing. IL-1beta serum levels were analyzed by a commercially available enzyme-linked immunosorbent assay.
Results:
Allele frequencies in women with endometriosis and controls were 76.6% and 76.8%, respectively, for the E1 allele (wild type) and 23.4% and 23.2%, respectively, for the E2 allele (polymorphic) (odds ratio 1.01; P > .99). The investigated polymorphism of the IL-1beta gene was not correlated with IL-1beta serum levels.
Conclusions:
We did not find an association between the IL1B exon 5 polymorphism, endometriosis, or increased serum IL-1beta levels.