Related Experiment Videos
Antimicrobial activity of endodontic sealers on Enterococcus faecalis
André K Mickel1, Tuan H Nguyen, Sami Chogle
1Department of Endodontics, Case Western Reserve University, School of Dentistry, Cleveland, OH 44106-4905, USA.
Journal of Endodontics
|April 19, 2003
Summary
This study assessed root canal sealers against Enterococcus faecalis, a bacteria linked to endodontic failures. Roth 811 demonstrated the most significant antimicrobial activity, while AH-Plus showed none.
Area of Science:
- Microbiology
- Endodontics
- Dental Materials Science
Background:
- Enterococcus faecalis is a resilient bacterium frequently implicated in endodontic treatment failures.
- Understanding the antimicrobial properties of root canal sealers is crucial for improving treatment outcomes.
Purpose of the Study:
- To evaluate and compare the in vitro antimicrobial efficacy of four commercially available root canal sealers against Enterococcus faecalis.
- To determine which sealer exhibits the greatest inhibitory effect on E. faecalis growth.
Main Methods:
- The study employed the Lawn technique, inoculating blood-agar plates with E. faecalis.
- Discs containing four sealers (Sealapex, Roth 811, Kerr EWT, AH-Plus) and an ampicillin control were placed on inoculated plates.
- Zones of inhibition were measured at 24 and 48 hours incubation at 37°C.
Main Results:
- A statistically significant difference (p < 0.05) in antimicrobial activity was observed among the tested sealers.
- Roth 811 exhibited the largest mean zone of inhibition (1.1 mm), followed by Sealapex (0.8 mm) and Kerr EWT (0.5 mm).
- AH-Plus demonstrated no measurable antimicrobial activity against E. faecalis.
Conclusions:
- Roth 811 possesses superior antimicrobial activity against E. faecalis compared to Sealapex, Kerr EWT, and AH-Plus.
- AH-Plus showed no efficacy in inhibiting E. faecalis growth under the study conditions.
- These findings suggest that sealer choice can significantly impact the control of E. faecalis in endodontic procedures.