The Ishikawa cells from birth to the present

Area of Science:

• Reproductive Biology
• Molecular Science
• Cancer Research

Background:

• The Ishikawa cell line is a well-differentiated human endometrial adenocarcinoma model.
• It expresses estrogen and progesterone receptors, making it valuable for reproductive and molecular research.
• Long-term culture can lead to dedifferentiation and loss of receptor expression in Ishikawa cells.

Purpose of the Study:

• To re-establish a high-quality, well-differentiated Ishikawa cell line.
• To ensure consistent research findings by maintaining cell line integrity.
• To provide a reliable supply of Ishikawa cells for basic research.

Main Methods:

• Establishment of new, well-differentiated cells from the parent Ishikawa cell line.
• Quality control measures to ensure receptor expression and differentiation status.
• Direct supply from the original investigator to maintain quality.

Main Results:

• Successfully established a new supply of well-differentiated Ishikawa cells.
• The new supply maintains essential characteristics, including receptor expression.
• Ensures the continued utility of the Ishikawa cell line for research.

Conclusions:

• Maintaining the quality and differentiation of established cell lines is critical.
• Direct management by the establishing investigator ensures cell line integrity.
• This work provides a reliable source of high-quality Ishikawa cells for the scientific community.