Determination of benomyl and 2-(4-thiazolyl) benzimidazole in plant tissues by high-performance liquid chromatography...

Methods for determining benomyl [methyl 1-(butylcarbamoyl)-2-benzimidazole carbamate] residues in plant tissues involve isolation of the residue by extraction with ethyl acetate, hydrolysis of the extracted residue to 2-aminobenzimidazole and final determination by high-performance liquid chromatography using a fluorimeter. 2-(4-Thiazolyl) benzimidazole is also determined by a similar procedure without hydrolysis. The sensitivities of these methods are 0.02 ppm for benomyl and 0.001 ppm for 2-(4-thiazolyl) benzimidazole. Recoveries of these compounds from various plant tissues were 90.5-102.9%. No interference with these methods from the plant tissues tested was found.