A two-step method for marking polypectomy sites in the colon and rectum
Related Experiment Video
Updated: Sep 26, 2026

The Pocket-Creation Procedure of Endoscopic Submucosal Dissection for Large Rectal Laterally Spreading Tumors
Published on: February 13, 2026
Akira Sawaki1, Tsuneya Nakamura, Takashi Suzuki
1Department of Gastroenterology, Aichi Cancer Center Hospital, Nagoya, Japan.
Background:
Intraoperative localization of polypectomy sites is critical for laparoscopic and open surgery, but conventional methods of marking the colorectum are sometimes unreliable. A new two-step method for marking polypectomy sites for identification during laparoscopic and open operations is described.
Methods:
Eighteen patients with postpolypectomy lesions necessitating preoperative marking were enrolled in this study. A physiologic saline solution was endoscopically injected into the submucosa to produce an artificial submucosal elevation (pseudopolyp). A small volume of India ink was then injected into the submucosal elevation (pseudopolyp) with a separate needle.
Results:
The two-step method was easily applied for all lesions without complication. At surgery, all lesions were immediately visualized.
Conclusion:
The two-step tattooing method proved to be easy, safe, and accurate for marking polypectomy sites.
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