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Related Experiment Videos

B cells in the aged: CD27, CD5, and CD40 expression.

Giuseppina Colonna-Romano1, Matteo Bulati, Alessandra Aquino

  • 1Grupppo di Studio sull'Immunosenescenza, Dipartimento di Biopatologia e Metodologie Biomediche, Università di Palermo, Corso Tukory, 211, 90134, Palermo, Italy. gcolonna@unipa.it

Mechanisms of Ageing and Development
|April 26, 2003
PubMed
Summary

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Aging alters B cell populations, decreasing CD5+ and CD40+ cells but increasing CD27+ cells. These changes, alongside increased NK cells, suggest altered antibody production in the elderly due to immune remodeling.

Area of Science:

  • Immunology
  • Gerontology
  • Cell Biology

Background:

  • Aging significantly impacts lymphocyte subpopulations, affecting immune function.
  • Specific B cell subsets, identified by CD27, CD5, and CD40 markers, play crucial roles in adaptive immunity.
  • CD27 marks memory B cells, CD5 is associated with B1 cells involved in T-cell-independent responses, and CD40 is vital for T-cell-dependent antibody production.

Purpose of the Study:

  • To investigate age-related changes in specific B cell subpopulations (CD27+, CD5+, CD40+).
  • To explore the implications of these changes on antibody production regulation in the elderly.
  • To examine the potential role of B and NK cell interactions in immune remodeling during aging.

Main Methods:

  • Flow cytometry analysis of peripheral blood lymphocytes.

Related Experiment Videos

  • Quantification of absolute numbers and percentages of B cells expressing CD27, CD5, and CD40 markers.
  • Comparison of B cell profiles between elderly individuals and younger controls (implied).

    • Main Results:

    • A decrease in the absolute number of CD5+ and CD40+ B cells was observed in the elderly.
    • CD27+ B lymphocytes showed only a marginal decrease in centenarians.
    • The percentage of CD5+ B cells decreased, while CD27+ B cells increased, with no significant change in CD40+ B cells.

    Conclusions:

    • Aging leads to distinct alterations in B cell subset distribution, characterized by reduced CD5+ and CD40+ cells and increased CD27+ cells.
    • These B cell changes, coupled with an increase in NK cells, suggest a remodeled immune system in the elderly.
    • Altered B and NK cell interactions may underlie the modified antibody production observed in aging individuals.