Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fungi allergens produced by solid-state fermentation process: optimization and allergen characterization.

Salah D M Hasan1, Walderez Gambale, Ricardo L Zollner

  • 1School of Chemical Engineering, State University of Campinas, PO Box 6066, 13083-970, Campinas-SP, Brazil.

Applied Biochemistry and Biotechnology
|May 2, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Renal tissue PLA2R and circulating anti-PLA2R detection in a Brazilian cohort: Precise immunostaining analysis in membranous nephropathy.

Clinical nephrology·2025
Same author

3D Printed Cartilage-Like Tissue Constructs with Spatially Controlled Mechanical Properties.

Advanced functional materials·2021
Same author

Rapid clinical recovery of a SARS-CoV-2 infected common variable immunodeficiency patient following the infusion of COVID-19 convalescent plasma.

Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology·2021
Same author

Mitotane liposomes for potential treatment of adrenal cortical carcinoma: <i>ex vivo</i> intestinal permeation and <i>in vivo</i> bioavailability.

Pharmaceutical development and technology·2020
Same author

Evaluation of the Influence of Process Parameters on the Properties of Resveratrol-Loaded NLC Using 2<sup>2</sup> Full Factorial Design.

Antioxidants (Basel, Switzerland)·2019
Same author

Chromoblastomycosis in the Amazon region, Brazil, caused by Fonsecaea pedrosoi, Fonsecaea nubica, and Rhinocladiella similis: Clinicopathology, susceptibility, and molecular identification.

Medical mycology·2019

Researchers developed potent allergenic extracts from Drechslera (Helminthosporium) monoceras using optimized solid-state fermentation. These extracts showed significant reactivity in atopic patients, indicating potential for allergy diagnosis and treatment.

Area of Science:

  • Mycology
  • Immunology
  • Biotechnology

Background:

  • Allergic diseases affect a significant portion of the population worldwide.
  • Accurate diagnosis and effective treatment of allergies rely on well-characterized allergenic extracts.
  • Drechslera (Helminthosporium) monoceras is a fungal species that can be allergenic.

Purpose of the Study:

  • To optimize solid-state fermentation for producing Drechslera (Helminthosporium) monoceras biomass.
  • To develop and characterize allergenic extracts from this fungal biomass.
  • To evaluate the allergenic potential of the extracts in atopic individuals.

Main Methods:

  • Solid-state fermentation of Drechslera (Helminthosporium) monoceras using wheat bran.
  • Statistical design for optimizing fermentation variables (pH, moisture).

Related Experiment Videos

  • Protein precipitation and polyphenol removal for extract purification.
  • Protein characterization (SDS-PAGE) and in vivo/in vitro immunological assays.

    • Main Results:

    • Optimized solid-state fermentation achieved a biomass yield of 1.43 mg/(g dry substrate·d) at pH 9.5 and 45.8% moisture.
    • Purified allergenic extracts contained proteins ranging from 16-160 kDa.
    • Cutaneous in vivo tests showed positive reactions in 40% of atopic patients.
    • Dot-blotting assays confirmed the reactivity of the extracts.

    Conclusions:

    • Optimized solid-state fermentation is an effective method for producing allergenic biomass from Drechslera (Helminthosporium) monoceras.
    • The developed allergenic extracts contain relevant allergenic proteins and demonstrate significant reactivity in atopic patients.
    • These findings support the potential use of these extracts in allergy diagnostics and immunotherapy.