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Cloning of structural gene of Deinococcus radiodurans UV-endonuclease beta
Shigeru Kitayama1, Issay Narumi, Tomoo Funayama
1Cell Physiology Laboratory, The Institute of Physical & Chemical Research, Wako, Saitama 350-0198, Japan.
Bioscience, Biotechnology, and Biochemistry
|May 2, 2003
Abstract:
To characterize its enzymic property we cloned and sequenced the gene of Deinococcus radiodurans encoding UV-endonuclease beta, an alternative enzyme to UvrABC repairing damaged DNA. Amino acid substitutions were found in UV-sensitive mutants. The putative amino acid sequence had some similarity with those of eukaryotic UV-endonucleases and with a sequence found in a protein data base of Bacillus subtilis.