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[The study on expression of caspase-1 in experimental brain contusion of different severity in rats]
Lu-yang Tao1, Xi-ping Chen, Mei Ding
1China Medical University, School of Forensic Medicine, Shenyang, P.R. China 110001. luyangtao@hotmail.com
Objective:
To investigate the expression of caspase-1 in different posttraumatic intervals and severity of brain injury.
Methods:
The study examined brain tissue samples of slight (n = 24), severe (n = 24) brain injury and control (n = 6) of rat, using immunohistochemical staining, western-blot and RT-PCR method.
Results:
Up-regulating of caspase-1 expression was found in tissue from traumatic brain injury compared with controls in early 1 hour after TBI, and lasted for 2 weeks. Caspase-1 cleaved after brain injury.
Conclusion:
These findings are the first time to demonstrate the study of different intense brain injury. The increasing of caspase-1 expression indicates that the TBI exists. The threshold area of caspase-1 positive cells is different in different severity of brain injury.