Related Experiment Video
Updated: Sep 26, 2026

Clarifying and Imaging Candida albicans Biofilms
Published on: March 6, 2020
Structure and biological activities of beta-glucans from yeast and mycelial forms of Candida albicans
Noriko N Miura1, Yoshiyuki Adachi, Toshiro Yadomae
1Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo 192-0392, Japan. miuranno@ps.toyaku.ac.jp
Abstract:
We have achieved the extraction of cell wall beta-glucan from the mycelial form of Candida albicans (C. albicans) IFO 0579 (M-CSBG) by using acetic acid, sodium hypochlorite (NaClO), and dimethylsulfoxide (DMSO) treatments. The yield of M-CSBG was significantly lower (7.5% from dried mycelial cells) than that of the yeast form from C. albicans IFO 1385 (Y-CSBG, 25.9% from dried yeast cells). The properties of M-CSBG were similar to those of Y-CSBG in terms of nuclear magnetic resonance (NMR) spectra and limulus reactivity. Molecular weight (Mw) of M-CSBG was slightly higher than that of Y-CSBG. Both Y-CSBG and M-CSBG induced the production of comparable amounts of macrophage inflammatory protein-2 (MIP-2), a chemotactic factor, from mouse peritoneal exudate cells (PEC) in vitro. These findings suggest that the structure and properties of CSBG from yeast and mycelial cells are similar to each other.
Related Concept Videos
Candidiasis
Antifungal Agents
Fungal Phylum Ascomycota
Fungal Group Zygomycota
Yeast Signaling
Overview of Fungi

