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High IgM production by human-human hybridoma HB4C5 cells cultured in microtubes
Takuya Sugahara1, Satomi Yano, Takeshi Sasaki
1Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan. mars95@agr.ehime-u.ac.jp
Bioscience, Biotechnology, and Biochemistry
|May 6, 2003
Abstract:
HB4C5 cells, a human-human hybridoma, were cultured in microtubes. After 24 h of cultivation, growth of the cells cultured in microtubes was 1.2- to 1.5-fold that in culture dishes or 96-well culture plates. The production of IgM was 2.6- to 3.3-fold that in the 96-well culture plates.