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Related Experiment Videos

Biological variation of soluble CD163.

H J Møller1, P Hyltoft Petersen, L Rejnmark

  • 1Department of Clinical Biochemistry, AAS Aarhus University Hospital, Denmark. hjmol@akh.aaa.dk

Scandinavian Journal of Clinical and Laboratory Investigation
|May 6, 2003
PubMed
Summary

Soluble CD163 (sCD163) exhibits significant biological variability, with day-to-day and diurnal fluctuations observed in healthy individuals. Analytical methods must ensure sufficient quality for reliable sCD163 monitoring in clinical settings.

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Area of Science:

  • Biochemistry
  • Immunology
  • Clinical Chemistry

Background:

  • A soluble plasma form of CD163 (sCD163) has been identified with anti-inflammatory properties.
  • Elevated sCD163 levels are observed in patients with myelo-monocytic leukemia and infections.
  • Understanding the biological variability and analytical quality of sCD163 assays is crucial for its clinical application.

Purpose of the Study:

  • To investigate the day-to-day and diurnal biological variability of sCD163 in healthy individuals.
  • To assess the analytical quality of a sandwich ELISA method for sCD163 measurement.
  • To determine the suitability of sCD163 for monitoring individual patient status.

Main Methods:

  • A sandwich ELISA was employed to measure sCD163 concentrations.

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  • 12 healthy individuals were studied to assess day-to-day and diurnal variations.
  • Analytical coefficients of variation (CV) and biological variation components were calculated.

    • Main Results:

    • The analytical CVs (within-run, between-run, total) were 3.6%, 4.8%, and 6.0%, respectively.
    • Within-subject biological variation was 9.0%, and between-subject variation was 35.9%.
    • A diurnal variation was observed, with 14% lower sCD163 levels at night; a low index of individuality (0.25) was found.

    Conclusions:

    • The analytical quality of the sCD163 assay is sufficient for clinical use.
    • Significant biological variability necessitates careful interpretation of sCD163 levels, especially for monitoring.
    • A critical difference of 30% is required to detect significant changes in serial sCD163 measurements for an individual.