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Related Experiment Videos

Field verification of Test-mate ChE.

Patterson W Taylor1, Brian J Lukey, Connie R Clark

  • 1U.S. Army Medical Research Institute of Chemical Defense, 3100 Ricketts Point Road, Aberdeen Proving Ground-EA, MD 21010-5400, USA.

Military Medicine
|May 8, 2003
PubMed
Summary

The Test-mate ChE accurately measures acetylcholinesterase (AChE) activity in blood samples exposed to soman under field conditions. This portable device offers reliable and reproducible results, especially for lower nerve agent doses.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Toxicology

Background:

  • Acetylcholinesterase (AChE) is a critical enzyme inhibited by organophosphorus nerve agents like soman.
  • Accurate and rapid determination of AChE activity is vital for assessing exposure and guiding medical treatment.
  • Field-deployable diagnostic tools are needed for immediate assessment in diverse environments.

Purpose of the Study:

  • To evaluate the Test-mate ChE device for quantifying blood AChE activity under realistic field conditions.
  • To assess the reliability and precision of the Test-mate ChE when exposed to varying levels of soman.
  • To determine the performance of the Test-mate ChE across different environmental temperatures during field deployment.

Main Methods:

  • Blood samples were spiked with known quantities of soman to simulate nerve agent exposure.

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  • The 520th Theater Army Medical Laboratory technicians performed blinded sample analysis during a field training exercise.
  • Measurements were conducted over six consecutive days, with repeated assessments on one day to ensure reproducibility.

    • Main Results:

    • The Test-mate ChE accurately identified all samples and quantified AChE activity.
    • The device demonstrated increased precision and reproducibility with smaller doses of soman.
    • Results remained reliable across the range of temperatures encountered during the field exercise.

    Conclusions:

    • The Test-mate ChE is a reliable and effective field instrument for determining blood AChE activity.
    • The device's performance is suitable for assessing nerve agent exposure in deployed settings.
    • The Test-mate ChE provides valuable data for rapid medical decision-making following potential chemical warfare agent exposure.