Related Experiment Video

Updated: Sep 26, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

Methods for the Detection of D-Amino-Acid Oxidase

Ryuichi Konno1

  • 1Department of Microbiology. Dokkyo University School of Medicine, Mibu, Tochigi 321-0293. Japan. konno@dokkyomed.ac.jp

Biological Procedures Online
|May 8, 2003
PubMed

Abstract:

Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described.

More Related Videos

Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System
16:41

Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System

Published on: November 5, 2013

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
12:47

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

Related Experiment Videos

Last Updated: Sep 26, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
11:01

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System
16:41

Detecting, Visualizing and Quantitating the Generation of Reactive Oxygen Species in an Amoeba Model System

Published on: November 5, 2013

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
12:47

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources

Published on: January 22, 2018

Related Concept Videos

Oxidations of Aldehydes and Ketones to Carboxylic Acids01:15

Oxidations of Aldehydes and Ketones to Carboxylic Acids

Oxidation of aldehydes and ketones results in the formation of carboxylic acids. Aldehydes, bearing hydrogen next to the carbonyl group, are easily oxidized compared to ketones. This is because an aldehydic proton can easily be abstracted during oxidation.
Aldehydes readily undergo oxidation in strong oxidizing agents such as potassium permanganate and chromic acid. The oxidation can also be carried out using mild oxidizing agents such as silver oxide. In fact, aldehydes can be easily oxidized...

Articles linked to this work by shared authors, journal, and citation graph.

Three-dimensional high-performance liquid chromatographic determination of serine, threonine and allothreonine enantiomers in the d-amino acid oxidase deficient mice and rats.

Journal of pharmaceutical and biomedical analysis·2026

Effect of D-amino acid metabolic enzyme deficiency on cancer development-diffuse large B-cell lymphoma onset and gene expression analyses in DASPO-knockout mice.

Amino acids·2024

AGGREGATELESS MUTANT DEFECTIVE IN SIGNALING AND RELAYING IN THE CELLULAR SLIME MOLD, DICTYOSTELIUM DISCOIDEUM.

Development, growth & differentiation·2023

Off-line two-dimensional LC-MS/MS determination of tryptophan enantiomers in mammalian urine and alteration of their amounts in d-amino acid oxidase deficient mice.

Journal of pharmaceutical and biomedical analysis·2022

Determination of phenylalanine enantiomers in the plasma and urine of mammals and ᴅ-amino acid oxidase deficient rodents using two-dimensional high-performance liquid chromatography.

Biochimica et biophysica acta. Proteins and proteomics·2020

Gut microbiota-derived D-serine protects against acute kidney injury.

JCI insight·2018

Identification and Validation of Genes Involved in Immune and Ferroptosis in Polycystic Ovarian Syndrome Using Bioinformatics and Experimental Approach.

Biological procedures online·2026

Huangjing Jiangzhi Granules Ameliorate Nonalcoholic Fatty Liver Disease by Activating the FOXA1-DERL1 Regulatory Axis to Suppress Lipid Accumulation, Endoplasmic Reticulum Stress, and Apoptosis.

Biological procedures online·2026

Therapeutic Mechanisms of Ruangan Huayu Formula in Liver Cirrhosis: Network Pharmacology, Molecular Docking and Experimental Validation.

Biological procedures online·2026

High Expression of SAMM50 Indicates Poor Clinical Prognosis in Hepatocellular Carcinoma and Represents a Potential Novel Biomarker.

Biological procedures online·2026

MKRN1 suppresses cuproptosis in colorectal cancer through ubiquitin-dependent regulation of SLC31A1.

Biological procedures online·2026

Ethacrynic Oxadiazole 6u Induces Apoptosis by Targeting mTOR/c-Flip Axis and Noxa in Lymphoma Cells.

Biological procedures online·2026

Evaluating Post-Analytical Stability of Blood Ethanol Samples Using Headspace-GC-FID.

Journal of analytical toxicology·2026

Lipid profiling of meat samples using surface extraction followed by multiple reaction monitoring profiling.

Analytical methods : advancing methods and applications·2026

Retention of Tritiated Alcohols by Zeolites Used in Indoor Air Sampling.

ACS omega·2026

Hydroxyethylcellulose- and Chitosan-Modified 3D-Printed Carbon Electrodes for Indole-3-Acetic Acid and Salicylic Acid Determination.

ACS omega·2026

Head-to-Head In Vitro Characterization of Novel HOCl-Targeted and Literature-Derived Fluorescent Probes Using Standardized Assay Conditions.

ACS omega·2026

Green Sorptive Sampling Coupled With UHPLC-IMS-MS and GC×GC-TOFMS for Characterization of Migrating Substances From Plastic Food Contact Materials.

Journal of separation science·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us