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Yeast Exoglycoproteins Produced Under NaCl-Stress Conditions as Efficient Cryoprotective Agents
1Culture Collection of Yeasts, Institute of Chemistry, Slovak Academy of Sciences. Dubravska cesta 9, 842 38 Bratislava. Slovakia. chememi@savba.sk
Biological Procedures Online
|May 8, 2003
Abstract:
Six extracellular yeast glycoproteins were prepared from three yeast species in osmotic equilibrium and unequilibrium environments and used as non-penetrating cryoadditives. Glycoproteins secreted by the strain Dipodascus australiensis into growth medium containing NaCl (8% w/v) were found to be the most effective cryoadditives. It was possible to use these glycoproteins alone (without DMSO as penetrating agent) for the cryoprotection of the studied yeasts.