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Published on: November 23, 2014
Expression of thrombospondin-derived 4N1K peptide-containing proteins in renal cell carcinoma tissues is associated
Yasuyoshi Miyata1, Shigehiko Koga, Kousuke Takehara
1Department of Urology, Nagasaki University Graduate School of Medical Science, Japan.
Purpose:
We have reported that the 4N1K peptide (KRFYVVMWKK) from thrombospondin (TSP) 1 has antiangiogenic activities. The goal of this study was to examine whether the expression of 4N1K-containing proteins correlates with reduced growth of human renal cell carcinoma (RCC).
Experimental Design:
We examined the expression of 4N1K-containing proteins and TSP1, microvessel density, proliferation index, and apoptotic index in 119 surgically excised RCC tissues by immunohistochemical techniques. The correlation between the above variables and clinicopathological features was analyzed statistically.
Results:
The antibody raised against the 4N1K peptide recognized protein fragments of matrix metalloproteinase 3-digested TSP1 and positively stained the sections of renal cancer tissues. These reactions disappeared when the antibody was preincubated with immobilized 4N1K peptide, suggesting that the reactions were 4N1K peptide specific. Although TSP1 expression did not correlate with various clinicopathological features and tumor size, all 4N1K-positive tumors were locally confined and of significantly smaller size (median, 3.3 cm; range, 2.0-4.4 cm) than 4N1K-negative tumors (median, 5.2 cm; range, 2.8-8.8 cm; P < 0.001). 4N1K-positive tumors exhibited significantly lower microvessel density and higher apoptotic index of tumor cells than 4N1K-negative tumors (P < 0.001 and P = 0.042, respectively).
Conclusions:
Our data suggest that expression of 4N1K-containing proteins in tumor tissues is associated with reduced angiogenesis and tumor growth; thus, it would be a potentially predictive marker for progression of RCC.
Insights
The 4N1K peptide, found in thrombospondin-1, is linked to reduced renal cell carcinoma (RCC) growth. Its presence in tumors correlates with smaller size, lower microvessel density, and increased apoptosis, suggesting it may predict RCC progression.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Thrombospondin-1 (TSP1) contains the 4N1K peptide, known for antiangiogenic properties.
- Renal cell carcinoma (RCC) is a significant malignancy with complex growth mechanisms.
Purpose of the Study:
- To investigate the correlation between 4N1K-containing proteins and the growth of human renal cell carcinoma (RCC).
- To determine if 4N1K expression serves as a predictive marker for RCC progression.
Main Methods:
- Immunohistochemical analysis of 4N1K-containing proteins, TSP1, microvessel density, proliferation, and apoptosis in 119 RCC tissues.
- Statistical correlation analysis between these markers and clinicopathological features.
Main Results:
- 4N1K-positive RCC tumors were significantly smaller and locally confined compared to 4N1K-negative tumors.
- 4N1K-positive tumors showed significantly reduced microvessel density and increased tumor cell apoptosis.
- TSP1 expression did not correlate with clinicopathological features or tumor size.
Conclusions:
- Expression of 4N1K-containing proteins in RCC tissues is associated with reduced angiogenesis and tumor growth.
- The 4N1K peptide may serve as a potential predictive biomarker for RCC progression.
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