Related Experiment Videos
[A new method of sputum pre-treatment for PCR preparation]
Takayuki Masaki1, Takayuki Ezaki
1Chemo-Sero Therapeutic Research Institute, Gifu University Graduate School of Medicine. masaki@infobears.ne.jp
Kekkaku : [Tuberculosis]
|May 13, 2003
Abstract:
A rapid sputum processing method was developed for PCR. As a protocol prepared for commercial kit is too much simplified, PCR inhibitor is not completely removed from sputum. We developed a new semi-alkaline protease method to dissolve sputum. From April 2001 to August 2001, 261 sputum samples were treated with our new method. Twenty-one cases (8.0%) were PCR positive and all the results perfectly coincided with the results of the conventional culture method.