Related Experiment Video
Updated: May 17, 2026
11:17
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Published on: June 1, 2017
Protein metabolism in Isoparorchis hypselobagri
Zeitschrift Fur Parasitenkunde (Berlin, Germany)
|April 30, 1976
Summary
This study investigated the protein content and nitrogen excretion of Isoparorchis hypselobagri. The fish parasite showed significant protein levels and specific nitrogenous waste products after starvation.
Area of Science:
- Parasitology
- Biochemistry
- Aquatic Biology
Background:
- Isoparorchis hypselobagri is an economically important fish parasite.
- Understanding its metabolic processes is crucial for parasite control and aquaculture.
Purpose of the Study:
- To determine the protein content of Isoparorchis hypselobagri.
- To quantify nitrogenous waste products (ammonia and uric acid) excreted by the parasite after a period of starvation.
Main Methods:
- Parasites were cultured in a sterile, non-nutrient medium for 12 hours under controlled aerobic conditions (30°C).
- Protein content was analyzed from dry tissue weight.
- Excreted nitrogen was measured as ammonia and uric acid percentages of total body nitrogen.
Main Results:
- Protein content ranged from 56.25% to 67.5%, averaging 63.5% ± 4.6% of dry weight.
- After 12 hours of starvation, 3.04% of total body nitrogen was excreted as ammonia.
- 1.6% of total body nitrogen was excreted as uric acid.
Conclusions:
- Isoparorchis hypselobagri possesses a high protein content.
- The parasite excretes nitrogen primarily as ammonia and secondarily as uric acid following starvation, indicating specific metabolic pathways.
More Related Videos
Related Concept Videos
01:01Production Efficiency
Net production efficiency (NPE) is the efficiency at which organisms assimilate energy into biomass for the next trophic level. Due to low metabolic rates and less energy spent on thermoregulatory processes, the NPE of ectotherms (cold-blooded animals) is 10 times higher than endotherms (warm-blooded animals).
01:40Overview of Metabolism
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
01:21Overview of Protein Metabolism
Proteins are broken down into amino acids during digestion. Unlike fats and carbohydrates, which are stored for later use, proteins are not. Instead, amino acids are either used to produce ATP through oxidation or contribute to the creation of new proteins for the growth and repair of the body. Any surplus amino acids from the diet are converted into glucose or triglycerides rather than excreted.
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...
01:15Metabolism of Chemolithotrophs
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation. However, because inorganic electron donors...
01:18Amino Acid Catabolism
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
01:29Biosynthesis of Lipids
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis pathway, which...