Antiglobulin test for titration of antilymphocyte globulin

An antiglobulin assay, derived from the indirect Coombs' reaction has been adapted to the titration of antilymphocyte globulin (ALG). Ten different ALG preparations have been investigated by this test and titres obtained are satisfactorily correlated with skin allograft survival in monkeys, rosette inhibition and mixed lymphocyte reaction inhibition. The antiglobulin test has also proved to be valuable for the monitoring of ALG-therapy in patients as well as for studying the mode of action of this potent immunosuppressive agent.