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Derivatization in mass spectrometry--1. Silylation
John M Halket1, Vladimir G Zaikin
1Drug Control Centre, King's College London, Franklin-Wilkins Building, Stamford Street, UK. john.halket@kcl.ac.uk
Summary
Silylation enhances the volatility and stability of organic compounds for mass spectrometry analysis. This review covers silylating agents, their mass spectral properties, and applications in compound identification and quantification.
Area of Science:
- Analytical Chemistry
- Organic Chemistry
Background:
- Derivatization is crucial for analyzing challenging compounds in mass spectrometry.
- Silylation is a key derivatization technique for improving analyte properties.
Purpose of the Study:
- To review advances in silylation for mass spectrometry.
- To detail the chemical aspects and mass spectral properties of silyl derivatives.
- To highlight the utility of silylation in analyzing organic and biologically-active compounds.
Main Methods:
- Review of silylation techniques and reagents.
- Analysis of mass spectral data of silyl derivatives.
- Discussion of gas chromatography-mass spectrometry (GC/MS) applications.
Main Results:
- Silylation significantly increases volatility, thermal stability, and chromatographic mobility.
- Mass spectral properties of silyl derivatives aid in structure determination and quantification.
- Comprehensive libraries and search systems are vital for identifying silyl derivatives.
Conclusions:
- Silylation is a powerful and versatile tool in mass spectrometry.
- The described methods and agents are widely applicable for analyzing diverse compounds.
- Effective use of silylation relies on understanding its chemical and spectral characteristics.