Related Experiment Videos
Outer membrane protein A (OmpA) activates human epidermal Langerhans cells.
Sylvie Godefroy1, Nathalie Corvaia, Daniel Schmitt
1INSERM U346, affilié CNRS, Hôpital E. Herriot, Lyon, France.
European Journal of Cell Biology
|May 20, 2003
Summary
Outer membrane protein A (OmpA) from Klebsiella pneumoniae activates human Langerhans cells (LC). This interaction enhances LC migration and immune response, suggesting OmpA recognition initiates antibacterial defense.
Area of Science:
- Immunology
- Microbiology
- Cell Biology
Background:
- Outer membrane protein A (OmpA) is a conserved protein in Enterobacteriaceae.
- Langerhans cells (LC) are key antigen-presenting cells in the skin and mucosa.
Purpose of the Study:
- To investigate the interaction between recombinant Klebsiella pneumoniae OmpA (kpOmpA) and human Langerhans cells.
- To determine if kpOmpA influences LC function and immune signaling.
Main Methods:
- Used biotinylated kpOmpA to study binding to isolated human LC.
- Assessed expression of MHC class II, CD86, and CCR7 on kpOmpA-treated LC.
- Evaluated LC migration through a reconstituted basement membrane.
- Measured the allostimulatory capacity of kpOmpA-treated LC.
Main Results:
- Biotinylated kpOmpA binds to human LC.
- kpOmpA up-regulates MHC class II, CD86, and CCR7 expression on LC.
- kpOmpA enhances LC migration towards MIP-3beta.
- kpOmpA-treated LC exhibit increased allostimulatory function.
Conclusions:
- kpOmpA binds to and activates human Langerhans cells.
- LC recognition of OmpA may be an initial step in the host's antibacterial response.
- This interaction highlights a potential mechanism for initiating immune defense against Enterobacteriaceae.