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Related Experiment Videos

A marked decrease in L-selectin expression by leucocytes in infants with Bordetella pertussis infection: leucocytosis

Greg Hodge1, Sandy Hodge, Corey Markus

  • 1Haematology Department, Women's and Children's Hospital, North Adelaide, South Australia, Australia. hodgeg@mail.wch.sa.gov.au

Respirology (Carlton, Vic.)
|May 20, 2003
PubMed
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Infants with whooping cough (pertussis) show increased white blood cells, particularly lymphocytes. A key finding is reduced L-selectin expression on these cells, potentially explaining leucocytosis and impacting immune response.

Area of Science:

  • Immunology
  • Pediatrics
  • Infectious Diseases

Background:

  • Infants with Bordetella pertussis infection exhibit unexplained lymphocytosis and leucocytosis.
  • Characterization of these specific lymphocyte populations is crucial for understanding pertussis pathogenesis.

Purpose of the Study:

  • To immunophenotypically characterize leucocytes in infants with pertussis.
  • To investigate the expression of various cell surface markers on leucocytes during pertussis infection.

Main Methods:

  • Multiparameter flow cytometry was used to analyze leucocytes from 11 infants with pertussis and control subjects.
  • A panel of monoclonal antibodies targeting activation, adhesion, costimulatory, and T-helper markers was employed.

Main Results:

Related Experiment Videos

  • Pertussis patients showed increased neutrophils, monocytes, T cells, B cells, and NK cells.
  • A significant decrease in L-selectin (CD62L) expression was observed on all leucocyte subgroups.
  • While some T-cell activation markers were unchanged, there was an increase in Th1-like CD4+ T cells.

Conclusions:

  • Down-regulation of L-selectin may impair leucocyte migration to infection sites and homing to lymphoid tissues.
  • The increase in Th1-like lymphocytes might be essential for an effective immune response to pertussis.
  • These findings offer a potential explanation for the observed leucocytosis in pertussis.