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Updated: Aug 7, 2026
09:01
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
Published on: March 16, 2011
Error-prone polymerase, DNA polymerase IV, is responsible for transient hypermutation during adaptive mutation in
Joshua D Tompkins1, Jennifer L Nelson, Jill C Hazel
1Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.
Journal of Bacteriology
|May 20, 2003
Abstract:
The frequencies of nonselected mutations among adaptive Lac(+) revertants of Escherichia coli strains with and without the error-prone DNA polymerase IV (Pol IV) were compared. This frequency was more than sevenfold lower in the Pol IV-defective strain than in the wild-type strain. Thus, the mutations that occur during hypermutation are due to Pol IV.
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