Selection of biochemically variant clones of human lymphoid cells

Two established clones of human lymphoid cells, Raji and P3HRI, could be cloned in semi-solid agarose-containing medium at high efficiency. Several other human lymphoid cell lines examined cloned poorly if at all under these conditions. Variant clones of Raji and P3HRI resistant to 6-thioguanine, and in the case of Raji also to either 8-azaadenine or 5-bromo-2'-deoxyuridine, were selected and characterized.