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Related Experiment Videos

External quality assurance schemes in flow cytometry.

T C Liu1

  • 1Department of Laboratory Medicine, National University Hospital, Lower Kent Ridge Road, Singapore.

The Southeast Asian Journal of Tropical Medicine and Public Health
|May 21, 2003
PubMed
Summary

External quality assurance schemes for clinical Flow Cytometry are underdeveloped, showing significant variability in results for key applications like CD34 enumeration. Challenges include unstable cell materials and inconsistent methods, hindering laboratory practice improvement.

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Area of Science:

  • Clinical laboratory diagnostics
  • Immunophenotyping
  • Cellular analysis

Background:

  • Quality assurance (QA) schemes are crucial for monitoring laboratory test performance.
  • External QA schemes in clinical Flow Cytometry are nascent and face significant challenges.
  • Major applications include lymphocyte subset enumeration, CD34 enumeration, and leukemia immunophenotyping.

Purpose of the Study:

  • To assess the current state of external quality assurance in clinical Flow Cytometry.
  • To identify challenges and variability in established Flow Cytometry applications.
  • To highlight the need for improved consensus and solutions in Flow Cytometry QA.

Main Methods:

  • Analysis of pilot external quality assurance schemes for CD34 enumeration and leukemia immunophenotyping.

Related Experiment Videos

  • Evaluation of results from lymphocyte subset enumeration surveys.
  • Identification of factors contributing to variability in Flow Cytometry QA.

    • Main Results:

    • Pilot schemes revealed a lack of consensus and wide practice divergence.
    • High coefficients of variation (20-200%) were observed in reported results.
    • Lymphocyte subset enumeration surveys also showed considerable variability despite longer establishment.

    Conclusions:

    • Significant variability and lack of consensus plague clinical Flow Cytometry QA.
    • Providing stable cellular materials and standardizing methodologies are key challenges.
    • Current QA schemes offer limited benefits for laboratory practice improvement, necessitating further development.