Determination of ambroxol in human plasma using LC-MS/MS

Area of Science:

• Analytical Chemistry
• Pharmacokinetics

Background:

• Accurate quantification of ambroxol in biological matrices is essential for pharmacokinetic and therapeutic drug monitoring.
• Existing methods may lack the sensitivity or selectivity required for precise ambroxol measurement in human plasma.

Purpose of the Study:

• To develop and validate a sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying ambroxol in human plasma.

Main Methods:

• Developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using domperidone as an internal standard.
• Plasma samples were extracted using diethyl ether under basic conditions and analyzed on a C(18) column.
• The mass spectrometer was operated in positive ion mode with multiple reaction monitoring (MRM) for detection.

Main Results:

• The developed LC-MS/MS method demonstrated high sensitivity and selectivity for ambroxol quantification.
• The method achieved a quantification limit of 0.2 ng/ml with accurate and precise results.
• The analytical column proved durable, lasting over 600 injections.

Conclusions:

• The validated LC-MS/MS method provides a reliable tool for the accurate and precise determination of ambroxol in human plasma.
• This method is suitable for application in pharmacokinetic studies and clinical settings requiring ambroxol level monitoring.