Related Experiment Video
Updated: Sep 25, 2026
Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
Published on: January 1, 2018
Adenosine-mediated synaptic depression and EPSP/spike dissociation following high potassium-induced depolarization in
Youn-Kwan Park1, Eun-Sheb Shim, Jae-In Oh
1Department of Neurosurgery, Korea University Guro Hospital, 80 Guro-dong, Guro-ku, Seoul 152-703, South Korea. ykapa@kumc.or.kr
Abstract:
Simultaneous recordings of orthodromic PS, fEPSP and antidromic PS revealed EPSP/spike (E-S) dissociation, indicating a conversion of input/output relations from early and brief excitability to a late and prolonged depression during the recovery from depolarization induced by high levels of potassium. E-S potentiation was partially attenuated by pre-treating the slices with BAPTA-AM and lidocaine and totally eliminated by a submaximal concentration of muscimol. The time lag for recovery was decreased by the GABA(A) antagonist and completely eliminated by the A(1) antagonist. From these observations, we conclude that Ca(2+) dependent inhibitory suppression is the main cause of a brief period of E-S potentiation, and accumulation of adenosine is the mechanism responsible for prolonged depression of synaptic transmission
