Related Experiment Video
Updated: Aug 3, 2026

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters
Published on: June 18, 2021
Spectral fitting: the extraction of crucial information from a spectrum and a spectral image
Colin Mailer1, Bruce H Robinson, Benjamin B Williams
1Center for EPR Imaging In Vivo Physiology, Department of Radiation and Cellular Oncology, University of Chicago, Illinois 60637, USA. cmailer@rover.uchicago.edu
Abstract:
A highly accurate line-width simulation computer program is used that can account for both high amplitude and frequency of the Zeeman modulation in an electron paramagnetic resonance (EPR) experiment. This allows for the overmodulation of EPR lines to increase signal-to-noise ratio (SNR) in EPR spectra and spectroscopic images, without any sacrifice in the determination of the intrinsic line width (1/gamma. T(2e)). The technique was applied to continuous-wave EPR spectroscopic images of a narrow, single-line trityl spin probe wherein a full EPR spectrum was extracted from each 3D spatial voxel. Typical improvements are a three- to fivefold increase in SNR in the high-gradient projections in the image and a reduction in the standard deviation (SD), by a factor of 3, of the line widths in the low-gradient domain. This method is a general one that is also applicable to the analysis of conventional (14)N or (15)N nitroxide spin probes.
Related Concept Videos
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
IR Spectrum
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0% (complete...
IR Spectrometers
IR Frequency Region: Fingerprint Region
The...
Applications of IR Spectroscopy: Overview

