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Updated: Sep 30, 2026
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
Target-sensitive aptasensor FRET readout using a fluorescent nucleobase surrogate for detection of ochratoxin A
Karley J L Zimmer1, Katharine E Lewis1, Dylan J Roby1
1Department of Chemistry & Toxicology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1. rmanderv@uoguelph.ca.
Abstract:
A target-sensitive FRET aptasensor platform is reported for ochratoxin A (OTA) detection. The intrinsic emission of OTA serves as the FRET donor to an internal fluorescent merocyanine nucleobase FRET acceptor. The platform achieves low nanomolar sensitivity and high selectivity over the nonchlorinated ochratoxin B (OTB) through binding-generated FRET readout.
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