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Related Experiment Videos

Pupal commitment and its hormonal control in wing imaginal discs.

Y Obara1, M Miyatani, Y Ishiguro

  • 1Department of Biology, Faculty of Science, Kanazawa University, Kakumamachi, 920-1192, Kanazawa, Japan

Journal of Insect Physiology
|May 29, 2003
PubMed
Summary

Forewing imaginal discs in silkworms (Bombyx mori) commit to pupation shortly after molting. Juvenile hormone loses influence, suggesting commitment begins before the final larval molt.

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Area of Science:

  • Developmental Biology
  • Insect Endocrinology
  • Molecular Biology

Background:

  • Imaginal discs are crucial for adult structure formation in insects.
  • Hormonal cues regulate metamorphosis timing and commitment.
  • Understanding pupal commitment in Bombyx mori is key to insect development research.

Purpose of the Study:

  • To determine the precise timing of pupal commitment in Bombyx mori forewing imaginal discs.
  • To investigate the roles of 20-hydroxyecdysone (20E) and juvenile hormone analog (JHA) in this process.

Main Methods:

  • Transplantation assays using fourth instar larvae.
  • In vitro culture of imaginal discs with and without hormones.
  • Hormonal treatments with 20E and JHA (Methoprene).

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Main Results:

  • Pupal commitment begins within 14 hours after ecdysis to the fifth instar.
  • In vitro cultured discs committed within 26 hours without hormones.
  • 20-hydroxyecdysone accelerated commitment, while JHA had no suppressive effect.

Conclusions:

  • Bombyx mori forewing discs lose sensitivity to juvenile hormone during the final larval molt.
  • 20-hydroxyecdysone is not required for commitment completion.
  • Pupal commitment may initiate prior to the last larval molt.