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[HPLC determination of 6-gingerol in Rhizoma Zingiberis Recens]
Wei-hao Wang1, Zhi-min Wang, Li-zhen Xu
1Institute of Chinese Meteria Medica, China Academy of Traditional Chinese Medicine, Bejing 100700, China.
Objective:
To determine the contents of 6-Gingerol in Rhizoma Zingiberis Recens.
Method:
HPLC method was used, with Alltech C18 column, acetonitrile-methol-water (43:5:52) as mobile phase with a flow rate of 0.8 mL.min-1, detecting wavelength 280 nm, and column temperature 35 degrees C.
Result:
Retained time of 6-gingerol was near 19 min, showing a good recovery (98.2%) and linear correlation (r = 0.9999). The contents of 6-gingerol were 1.35-2.87 mg.g-1, and the water contents were 70.4-85.5% mL.g-1 in Rhizoma Zingiberis Recens.
Conclusion:
The method is appropriate for the determination of 6-gingerol in Rhizoma Zingiberis Recens. Gingerol can be used as a chemical marker of the quality control of Rhizoma Zingiberis Recens.
