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[Determination of imperatorin in tetraploidy radix Angelicae dahuricae]
Fei Peng1, Sheng Zhang, Ta-si Liu
1Hunan College of TCM, Changsha 410004, Hunan, China.
Objective:
To determine the content of imperatorin in the tetraploidy radix angelicae dahuricae, and compare it with the original diplontic varites.
Method:
The chromatographic method was carried out on Nova-pak (4.6 mm x 200 mm, 5 microns) column with acetonitrile-water solution as a mobile phase. The flow rate was 1 ml.min-1. the detection wavelength was at 248 nm, and the column temperature was 25 degrees C.
Result:
The eontent of was imperatorin in the tetraploidy radix 0.460% and 0.225% imperatorin in the diplontic species.
Conclusion:
The content of the mainly active constituent in the tetraploid is double to what it is in the original diplontic species.
