Related Experiment Video
Updated: Jul 31, 2026

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
[Determination of shikimic acid in fruit of Illiciaceae plants by HPLC with diode-array detection]
Objective:
To establish the determination method of Shikimic acid.
Method:
Using HPLC method as the determination method. The separation was performed in a SiO2-NH2 column with a mobile phase of Acetonitrile-2% H3PO4 water solution (95:5); The sample wavelength was 213 nm, reference wavelength 300 nm.
Result:
The average collection was 98.5%, RSD 1.67% (n = 5).
Conclusion:
This method is suitable for the determination of Shkimic acid in herb medicines and preparation containing shikimic acid.
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