[RAPD analysis on different varieties of Rehmannia glutinosa]

Jing-li Cheng¹, Lu-qi Huang, Ai-juan Shao

¹Hospital of XuanWu Traditional Chinese Medicine, Beijing 100050, China.

Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica

|June 5, 2003

Abstract

Objective:

To provide molecular evidences for its breeding by studying the genetic relationship among varieties of Rehmannia glutinosa.

Method:

Nineteen varieties were detected by Randomly Amplified Polymorphic DNA(RAPD) markers.

Result:

The 20 selected primers produced 163 bands, among which 114(69.9%) were polymorphic. A DNA molecular dendrogram was established based on Hierarchical cluster analysis of 163 DNA bands amplified by 20 primers, which divided the 19 varieties into four groups: Group Beijing, Group 85-5, Group Guolimao and the other Group.

Conclusion:

8 varieties of Group Beijing have a close genetic relationship, and so have varieties of Group 85-5, which provides information for Rehmannia glutinosa's breeding.

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Objective:

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Result:

Conclusion:

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