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[HPLC determination of two flavonoid compounds in Psoralea corylifolia]
Rui-min Lin1, Da-wei Wang, Zhi-li Xiong
1Department of Chemistry, Anshan Teacher's College, Anshan 114005, Liaoning, China.
Objective:
To determine two flavonoid compounds in Psoralea corylifolia L. (PC) simultaneously with HPLC method.
Method:
Bavachin and corylin isolated from PC and purified in our laboratory were used as the reference compounds. The HPLC separation was carried out on an Techsphere ODS column using mobile phase consisting of a mixture of methanol and 20 mmol.L-1 ammonium acetate buffer pH 4.0 (67:33), and the UV detection wavelength was 240 nm.
Result:
Simultaneous determination of bavachin and corylin was achieved. The linear range was 1.25-20 micrograms.mL-1 for both bavachin and corylin. The average recovery of bavachin and corylin was 94.9% and 96.2%, and RSD was 3.1% and 3.6% respectively.
Conclusion:
This is the first report on simultaneous determination of bavachin and corylin in PC with satisfactory accuracy and reproducibility.