Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Connectivity-based ab initio phasing at different solvent levels.

Andrei Fokine1, Natalia Lunina, Vladimir Lunin

  • 1LCM3B, UMR 7036 CNRS, Faculté des Sciences, Université Henry Poincaré Nancy I, 54506 Vandoeuvre-lés-Nancy, France.

Acta Crystallographica. Section D, Biological Crystallography
|June 5, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In situ structures of the portal-neck-tail complex of bacteriophage T4 inform a viral genome positioning mechanism.

Nature communications·2026
Same author

Cryo-EM structures of bacteriophage T4 portal-neck assembly intermediates reveal a viral genome retention mechanism.

Nature communications·2026
Same author

Cryo-EM structures of bacteriophage T4 portal-neck connector complexes reveal a viral genome retention mechanism involving a host component.

Research square·2025
Same author

Capsid structure of bacteriophage ΦKZ provides insights into assembly and stabilization of jumbo phages.

Nature communications·2024
Same author

Universal parameters of bulk-solvent masks.

Acta crystallographica. Section A, Foundations and advances·2024
Same author

Structure of <i>Vibrio</i> Phage XM1, a Simple Contractile DNA Injection Machine.

Viruses·2023
Same journal

Structural insights into the synthesis of FMN in prokaryotic organisms.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Native sulfur/chlorine SAD phasing for serial femtosecond crystallography.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Serial crystallographic analysis of protein isomorphous replacement data from a mixture of native and derivative microcrystals.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

The first crystal structure of the peptidase domain of the U32 peptidase family.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis.

Acta crystallographica. Section D, Biological crystallography·2015
See all related articles

The connectivity-based phasing method successfully determined low-resolution structures of a tRNA-synthetase complex by varying solvent levels. These images accurately reflect the solvent content within the crystal.

Area of Science:

  • Crystallography
  • Structural Biology
  • Biophysics

Background:

  • Accurate structure determination is crucial for understanding molecular mechanisms.
  • Neutron diffraction offers unique contrast for biological macromolecules.
  • Solvent accessibility influences crystal packing and diffraction data.

Purpose of the Study:

  • To apply connectivity-based phasing to neutron diffraction data.
  • To investigate the effect of varying solvent content on crystal structure.
  • To validate the connectivity-based phasing method for macromolecular complexes.

Main Methods:

  • Collected three neutron diffraction datasets from the same tRNA(Asp)-aspartyl-tRNA synthetase complex crystal.
  • Varied the D(2)O/H(2)O ratio across datasets to alter solvent contrast.

Related Experiment Videos

  • Applied connectivity-based phasing to analyze the diffraction data.

    • Main Results:

    • Achieved low-resolution structural images of the complex.
    • Observed perfect correlation between the obtained images and solvent levels.
    • Demonstrated the utility of connectivity-based phasing in variable contrast conditions.

    Conclusions:

    • Connectivity-based phasing is effective for macromolecular structure determination.
    • Neutron diffraction with varying solvent contrast provides insights into crystal composition.
    • The method accurately visualizes solvent distribution within macromolecular crystals.