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Updated: Aug 10, 2026

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
Published on: January 10, 2018
A single mutation of restriction endonuclease EcoRII led to a new crystal form that diffracts to 2.1 A resolution
Xiaoyin E Zhou1, Yujun Wang, Monika Reuter
1Laboratory for Structural Biology, Department of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USA.
Abstract:
R88A, a mutant of the type IIE restriction endonuclease EcoRII, has been crystallized in space group P2(1), with unit-cell parameters a = 58.7, b = 92.4, c = 88.3 A, beta = 108.1 degrees. There are two monomers in the asymmetric unit and the solvent content is estimated to be 50% by volume. The crystals diffract to 2.1 A resolution, which is much higher than that of the wild type, which diffracted to 2.8 A resolution. The mutant crystals have been used in the identification of an excellent heavy-atom derivative.
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