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Determination of uracil in 5-fluorouracil substance by high-performance liquid chromatography

H Kazoka1

  • 1Latvian Institute of Organic Synthesis, 21 Aizkraukles Street, LV-1006 Riga, Latvia. helena@osi.lv

Journal of Chromatography. A
|June 5, 2003
PubMed

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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process: