Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Heparan sulfate-FGF10 interactions during lung morphogenesis.

Konstantin I Izvolsky1, Deana Shoykhet, Yu Yang

  • 1Pulmonary Center, Department of Medicine, Boston University School of Medcine, MA 02118, USA.

Developmental Biology
|June 5, 2003
PubMed
Summary

Heparan sulfates (HS) modulate fibroblast growth factor 10 (FGF10) signaling crucial for lung development. Specific O-sulfated HS patterns in the lung mesenchyme and epithelium are essential for FGF10-mediated lung bud formation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Future of the COVID-19 Pandemic: How Good (or Bad) Can the SARS-CoV2 Spike Protein Get?

Cells·2022
Same author

Lipid Raft Association Stabilizes VEGF Receptor 2 in Endothelial Cells.

International journal of molecular sciences·2021
Same author

Hypoxia Induced Heparan Sulfate Primes the Extracellular Matrix for Endothelial Cell Recruitment by Facilitating VEGF-Fibronectin Interactions.

International journal of molecular sciences·2019
Same author

Heparin potentiates Avastin-mediated inhibition of VEGF binding to fibronectin and rescues Avastin activity at acidic pH.

The Journal of biological chemistry·2019
Same author

Ecosystem Traits Linking Functional Traits to Macroecology.

Trends in ecology & evolution·2018
Same author

Dual-wavelength end-pumped Rb-Cs vapor lasers.

Applied optics·2018

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Fibroblast growth factor 10 (FGF10) signaling via FGFR2b is vital for lung development.
  • Heparan sulfates (HS) are known modulators of growth factor signaling, but their role in vertebrate lung development is unclear.

Purpose of the Study:

  • To map HS expression in the developing lung.
  • To investigate how HS interactions with FGF10-FGFR2b influence lung morphogenesis.
  • To elucidate the role of HS sulfation patterns in FGF10-mediated lung budding.

Main Methods:

  • Mapping of HS expression in embryonic lungs.
  • Analysis of HS-FGF10-FGFR2b interactions using embryonic lung cultures.
  • Experiments with selectively sulfated heparins.

Related Experiment Videos

  • Assessment of gene expression and lung pattern formation.

    • Main Results:

    • Specific O-sulfated HS are dynamically expressed in the lung mesenchyme near Fgf10 expression.
    • Highly sulfated HS are found in the basement membranes of branching epithelial tubules.
    • Disrupting HS gradients or altering HS sulfation impairs FGF10 signaling and lung pattern formation.
    • O-sulfated HS groups are critical for FGF10 signaling activation during lung bud formation.

    Conclusions:

    • Developmentally regulated regional patterns of HS sulfation critically influence FGF10's ability to induce lung budding.
    • HS 6-O-sulfotransferase expression is localized to the tips of branching tubules.
    • HS sulfation patterns are essential for FGF10-mediated lung morphogenesis.