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Preservation of yeast cell morphology for scanning electron microscopy using 3.28-micro m IR laser irradiation
Jan Sunner1, Recep Avci, Lee Richards
1Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA. sunner@chemistry.montana.edu
Journal of Microbiological Methods
|June 5, 2003
Abstract:
As an alternative to conventional fixation procedures for scanning electron microscopy (SEM) analysis, yeast cells (Saccharomyces cerevisiae) were irradiated in ambient air, with an intense 3.28-micro m IR laser pulse. The morphology of the irradiated cells was well preserved, while nonirradiated control cells were severely shriveled.