Related Experiment Video
Updated: Sep 25, 2026
The Establishment of a Murine Mandibular Molar Extraction Socket Healing Model
Published on: January 13, 2023
A murine model of cholesteatoma-induced bone resorption using autologous dermal implantation
Holger Sudhoff1, Yvonne Liebehenz, Jessica Aschenbrenner
1Department of Otorhinolaryngology-Head and Neck Surgery, University of Bochum, Germany. Holger.Sudhoff@ruhr-uni-bochum.de
Objective:
To investigate a novel murine model for dermal implant-induced osteolysis analogous to bone resorption observed in middle ear cholesteatoma.
Study Design:
Animal experiment.
Methods:
We placed autologous dermal implants on the surface of mouse calvaria. The calvaria were examined at days 1, 3, 5, 7, and 14 after implantation by histological study and tartrate-resistant acid phosphatase immunohistochemical processing to detect osteoclasts.
Results:
Dermal implants showed a significantly increased osteoclast density compared with control samples. The dermal implant tissue remained viable and produced a robust, localized inflammatory osteolytic response on the adjacent calvarial surface. Osteoclasts were predominantly found on the surface of the calvarium with the greatest osteoclast density seen at 5 to 7 days after implantation.
Conclusion:
The mouse model is expected to be a useful tool for investigating the pathogenesis of localized inflammatory bone resorption related to cholesteatoma.
More Related Videos
05:10Improved Methodology for Studying Postnatal Osteogenesis via Intramembranous Ossification in a Murine Bone Marrow Injury Model
Published on: February 7, 2025
11:31Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate
Published on: October 31, 2012