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[Real-time imaging of local Ca2+ signaling in single neurons]
1Ca2+ Oscilation Project, Japan Science and Technology Corporation, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. tnakamu@ims.u-tokyo.ac.jp
Abstract:
In addition to the multiple mechanisms of the intracellular calcium mobilizing pathways, neurons possess multiple functional compartments such as the soma, the axon, the dendrites, and the spines. In this article, technical procedures and tips are described to measure local calcium signaling in response to neuronal excitation in single neurons.